Locus-specific editing of histone modifications at endogenous enhancers
نویسندگان
چکیده
منابع مشابه
Crosstalk between histone modifications maintains the developmental pattern of gene expression on a tissue-specific locus.
Genome wide studies have provided a wealth of information related to histone modifications. Particular modifications, which can encompass both broad and discrete regions, are associated with certain genomic elements and gene expression status. Here we focus on how studies on the beta-globin gene cluster can complement the genome wide effort through the thorough dissection of histone modifying p...
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The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adopted for a wide range of biological applications including genome editing. In some cases, dissection of genome functions requires allele-specific genome editing, but the use of CRISPR for this purpose has not been studied in detail. In this study, using the p16INK4a gene in HCT116 as a model locus, we inve...
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MyoD1 is a key regulator that orchestrates skeletal muscle differentiation through the regulation of gene expression. Although many studies have focused on its role in transcriptional control at gene promoters, less is known regarding the role of MyoD1 in the assembly of active enhancers. Here, we discuss novel data that point to the ability of MyoD1 to mediate the assembly of active enhancers ...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2013
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt.2701